Protein Extraction and Purification

Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit

Easily capture and affinity purify binding partners of GST-tagged fusion proteins with these glutathione (GSH) agarose beads and complete reagent set.

Cytiva Glutathione Sepharose™ 4 Fast Flow  Media

Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Cytiva His GraviTrap™ Columns

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

Thermo Scientific™ Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.5 mL

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.5 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Thermo Scientific™ DNase I Solution (1 unit/μL), RNase-free

Thermo Scientific™ DNase I Solution (1 unit/μL), RNase-free

Cleave and degrade unwanted single- and double-stranded DNA

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Thermo Scientific™ Pierce™ Disposable Column Trial Pack

Thermo Scientific™ Pierce™ Disposable Column Trial Pack

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

Thermo Scientific™ DNase I Solution (2500 U/mL)

Thermo Scientific™ DNase I Solution (2500 U/mL)

Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.

Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose

Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce™ Centrifuge Columns, 2 mL

Thermo Scientific™ Pierce™ Centrifuge Columns, 2 mL

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

Thermo Scientific™ Pierce™ Spin Columns - Screw Cap

Thermo Scientific™ Pierce™ Spin Columns - Screw Cap

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Thermo Scientific™ Lysozyme

Thermo Scientific™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts

Cytiva Sephadex™ G75 Media

Cytiva Sephadex™ G75 Media

Suitable for gel filtration

Cytiva HisTrap™ FF Crude Prepacked Columns

Cytiva HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Cytiva HisTrap™ HP Prepacked Columns

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Cytiva Superdex™ 200 Prep Grade

Cytiva Superdex™ 200 Prep Grade

Produce separation medium with high selectivity and resolution.  Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D

Invitrogen™ 24-Well Magnetic Separator

Invitrogen™ 24-Well Magnetic Separator

Contains six neodymium magnets that aligned with wells of a 24-well plate for efficient processing of DNA bound to magnetic beads.

Invitrogen™ Quant-iT™ Protein Assay Kit

Invitrogen™ Quant-iT™ Protein Assay Kit

For easy and accurate protein quantitation

Thermo Scientific™ Pierce™ Centrifuge Columns, 10 mL

Thermo Scientific™ Pierce™ Centrifuge Columns, 10 mL

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

Thermo Scientific™ Pierce™ Glutathione Agarose

Thermo Scientific™ Pierce™ Glutathione Agarose

Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.

Thermo Scientific™ Pierce™ Spin Columns - Snap Cap

Thermo Scientific™ Pierce™ Spin Columns - Snap Cap

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL).

Thermo Scientific™ HisPur™ Ni-NTA Resin

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Sartorius Vivacon™ 500 DNA Concentrator

Sartorius Vivacon™ 500 DNA Concentrator

For up to 500 μL, Vivacon™ 500 are centrifugal units for fast DNA concentration and buffer exchange of 500 μL samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart™ membranes results in high recoveries from dilute samples.

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Invitrogen™ DynaMag™-15 Magnet

Invitrogen™ DynaMag™-15 Magnet

Optimized for efficient cell isolation using all types of Dynabeads™ (range 1–4.5 μm in diameter) in standard 15 mL lab tubes.

Thermo Scientific™ Detergent Removal Spin Columns & Plates

Thermo Scientific™ Detergent Removal Spin Columns & Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 μg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 μg/mL.

Invitrogen™ Magnetic Stand-96

Invitrogen™ Magnetic Stand-96

The stand has clear polyacryl base in standard SBS 96-well microplate footprint and uses 24 extremely strong permanent magnets for fast, easy magnetic separation in little as 30 seconds.

Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.

Thermo Scientific™ HA Synthetic Peptide

Thermo Scientific™ HA Synthetic Peptide

HA Synthetic peptide 26184 from Thermo Scientific™ specifically detects HA in samples, and it is validated for Control

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